Other
Part:BBa_K4143336:Design
Designed by: Michigan Synthetic Biology Team Group: iGEM22_Michigan (2022-09-20)
Antimicrobial Pepide: HBCM2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We codon-optimized a sequence found in literature for the e.coli B21 strain we planned on using to produce the plasmid.
Source
HBCM2 is a cecropin melittin hybrid peptide. Cecropin is an AMP derived from the hemolymph of the cecropia moth, while melittin is a peptide derived from bee venom.